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Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues
Author(s) -
Fezza Filomena,
Bisogno Tiziana,
Minassi Alberto,
Appendino Giovanni,
Mechoulam Raphael,
Di Marzo Vincenzo
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02341-4
Subject(s) - chemistry , endocannabinoid system , ether , mass spectrometry , chromatography , anandamide , isotope dilution , cannabinoid , hippocampus , chemical ionization , biochemistry , ionization , biology , cannabinoid receptor , organic chemistry , endocrinology , receptor , agonist , ion
The occurrence of the novel proposed endocannabinoid, noladin ether (2‐arachidonyl glyceryl ether, 2‐AGE) in various rat organs and brain regions, and its inactivation by intact C6 glioma cells, were studied. 2‐AGE was measured by isotope dilution liquid chromatography‐atmospheric pressure chemical ionization‐mass spectrometry, with a detection limit of 100 fmol. A compound with the same mass and chromatographic/chemical properties as 2‐AGE was found in whole brain, with the highest amounts in the thalamus and hippocampus. Synthetic [ 3 H]2‐AGE was inactivated by intact rat C6 glioma cells by a time‐ and temperature‐dependent process consisting of cellular uptake and partial incorporation into phospholipids. Further data suggested that 2‐AGE is taken up by cells via the anandamide/2‐arachidonoyl glycerol (2‐AG) membrane transporter(s), and biosynthesized in a different way as compared to 2‐AG.