Premium
Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray 1
Author(s) -
Takeuchi Hideaki,
Fujiyuki Tomoko,
Shirai Kenichi,
Matsuo Yuko,
Kamikouchi Azusa,
Fujinawa Yumi,
Kato Azusa,
Tsujimoto Atsumi,
Kubo Takeo
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02319-0
Subject(s) - complementary dna , mushroom bodies , biology , gene , dna microarray , differential display , microarray , microarray analysis techniques , microbiology and biotechnology , gene expression , genetics , drosophila melanogaster
To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display‐positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5‐trisphosphate (IP 3 ) 5‐phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large‐type Kenyon cells, suggesting that IP 3 ‐mediated Ca 2+ signaling is enhanced in these neurons.