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The major light‐harvesting pigment protein of Acaryochloris marina
Author(s) -
Chen Min,
Quinnell Rosanne G,
Larkum Anthony W.D
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02315-3
Subject(s) - chemistry , chromatography , chlorophyll , pigment , chlorophyll a , thylakoid , light harvesting complex , botany , biochemistry , photosystem ii , biology , chloroplast , photosynthesis , organic chemistry , gene
The major light‐harvesting protein complex containing chlorophyll (Chl) d was isolated from Acaryochloris marina thylakoid membranes. Isolation was achieved by detergent solubilisation followed by separation on 6–40% sucrose gradients using ultracentrifugation. The best Chl d yield (70%) used 0.3% dodecyl maltoside, 0.15% octyl glucoside, 0.05% zwittergent 3‐14 with the detergent:total Chl d ratio around 10:1 (w/w). Characterisation of the light‐harvesting pigment protein complex (lhc) involved non‐denaturing electrophoresis, SDS–PAGE, absorbance and fluorescence spectroscopy. The main polypeptide in the lhc was shown to be ca. 34 kDa and to contain Chl d and Chl a , indicating that the Acaryochloris lhc is similar to that of prochlorophytes. The Chl a level varied with the culture conditions, which is consistent with previous findings.