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Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27
Author(s) -
Miyazaki Junichi,
Kobashi Nobuyuki,
Fujii Tomoyuki,
Nishiyama Makoto,
Yamane Hisakazu
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02290-1
Subject(s) - thermus thermophilus , operon , mutant , lysine , biochemistry , biosynthesis , chemistry , gene , arginine , escherichia coli , genetics , biology , amino acid
We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ – orfC – orfD – argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK . Purified LysK protein has deacetylating activities for both N 2 ‐acetyllysine and N 2 ‐acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus .