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Sucrose is involved in the diazotrophic metabolism of the heterocyst‐forming cyanobacterium Anabaena sp
Author(s) -
Curatti Leonardo,
Flores Enrique,
Salerno Graciela
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02283-4
Subject(s) - sucrose , diazotroph , biochemistry , cyanobacteria , disaccharide , biology , metabolism , anabaena , nitrogenase , microbiology and biotechnology , chemistry , nitrogen fixation , bacteria , genetics
Sucrose synthase (SuS) expression was studied in the filamentous, nitrogen‐fixing cyanobacterium Anabaena sp. PCC 7119. SuS activity, SusA polypeptide, and susA mRNA levels were lower in cells cultured diazotrophically than in the presence of combined nitrogen. An insertional susA mutant presented a dramatic increase in sucrose levels, whereas the disaccharide was not detectable in a susA overexpressing strain, indicating that SusA is involved in the cleavage of sucrose in vivo. Diazotrophic growth was impaired in the susA overexpressing strain, suggesting a role for sucrose in diazotrophic metabolism and the involvement of SusA in the control of carbon flux in the N 2 ‐fixing filament.