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Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage‐gated K + channel Kv2.1 1
Author(s) -
Sano Yorikata,
Mochizuki Shinobu,
Miyake Akira,
Kitada Chika,
Inamura Kohei,
Yokoi Hiromichi,
Nozawa Katsura,
Matsushime Hitoshi,
Furuichi Kiyoshi
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02267-6
Subject(s) - protein subunit , immunoprecipitation , voltage gated ion channel , ion channel , transfection , cloning (programming) , chemistry , electrophysiology , biophysics , biology , microbiology and biotechnology , biochemistry , gene , neuroscience , receptor , computer science , programming language
We report identification and characterization of Kv6.3, a novel member of the voltage‐gated K + channel. Reverse transcriptase‐polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage‐gated ion channel. When Kv6.3 and Kv2.1 were co‐expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co‐transfected cells. These results indicate that Kv6.3 is a novel member of the voltage‐gated K + channel which functions as a modulatory subunit.