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The characterization of the human Siah‐1 promoter 1
Author(s) -
Maeda Ayaka,
Yoshida Tatsushi,
Kusuzaki Katsuyuki,
Sakai Toshiyuki
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02265-2
Subject(s) - tata box , microbiology and biotechnology , reporter gene , biology , promoter , gene , transfection , plasmid , gene expression , genetics
Siah‐1, the human homologue of Drosophila seven in absentia, is related to apoptosis and tumor suppression. Although it was reported that the expression of Siah‐1 is induced by p53 and p21/WAF1, little is known about the transcriptional regulation of the Siah‐1 gene. To investigate the transcriptional regulation, we isolated and sequenced the genomic fragment of the Siah‐1 promoter region. The Siah‐1 promoter has no typical TATA box or CCAAT box. Transient transfection assays using reporter plasmids in which the promoter region of the Siah‐1 gene was deleted or mutated showed that one Sp1 site was responsible for the basal promoter activity. In Northern blotting analysis, the expression of the Siah‐1 gene was upregulated by p53, but activation of the reporter plasmid by the p53 co‐transfection assay was not shown, suggesting that a p53 responsive element does not exist in the promoter region we examined in this study but might be present in another region.