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Participation of peroxynitrite in oxidative modification of LDL by aqueous extracts of cigarette smoke
Author(s) -
Yamaguchi Yu,
Kagota Satomi,
Haginaka Jun,
Kunitomo Masaru
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02263-9
Subject(s) - peroxynitrite , chemistry , oxidative phosphorylation , cigarette smoke , aqueous solution , posttranslational modification , third hand smoke , smoke , biochemistry , organic chemistry , sidestream smoke , toxicology , biology , superoxide , enzyme
Aqueous extracts of cigarette smoke (CSE) can oxidatively modify plasma low‐density lipoprotein (LDL). The aim of the present study was to elucidate the participation of peroxynitrite in LDL oxidation. When LDL was incubated with CSE, its oxidative modification was dependent on time and concentration. It could be effectively prevented by vitamin E, partially by superoxide dismutase, but hardly by catalase, mannitol and metal chelators. CSE also increased the 3‐nitrotyrosine content in LDL. A similar increase of 3‐nitrotyrosine occurred after incubation of LDL with a peroxynitrite generating agent, 3‐morpholinosydnonimine, thus suggesting that prominent pro‐oxidants in CSE are peroxynitrite‐generating species.