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ADP‐evoked phospholipase C stimulation and adenylyl cyclase inhibition in glioma C6 cells occur through two distinct nucleotide receptors, P2Y 1 and P2Y 12
Author(s) -
Czajkowski Rafal̵,
Lei Lingsheng,
Sabal̵a Pawel̵,
Barańska Jolanta
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02255-x
Subject(s) - adenylyl cyclase , p2y receptor , phospholipase c , adcy9 , receptor , apyrase , purinergic receptor , ppads , biology , chemistry , biochemistry , microbiology and biotechnology
In this study we characterized the subtypes of nucleotide P2Y receptors that respond to ADP in glioma C6 cells. Direct visualization of phosphatidylinositol 4,5‐bisphosphate at the cell surface revealed that extracellular ADP activates phospholipase C (PLC). Knock‐down of P2Y 1 receptor with antisense oligonucleotide, as well as treatment with MRS2179 and pyridoxal‐phosphate‐6‐azophenyl‐2′,4′‐disulfonic acid (P2Y 1 antagonists), attenuates receptor‐mediated PLC activity. Adenylyl cyclase inhibition by ADP remains unchanged under these conditions. Reverse transcription‐PCR analysis showed that P2Y 12 receptor is expressed in C6 cells. We therefore conclude that, in glioma C6 cells, two P2Y receptor subtypes are present: P2Y 1 , coupled to PLC, and P2Y 12 , negatively coupled to adenylyl cyclase.