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Murine T cells expressing high activity of prolyl endopeptidase are susceptible to activation‐induced cell death
Author(s) -
Odaka Chikako,
Mizuochi Toshiaki,
Shirasawa Takuo,
Morain Philippe,
Checler Fredéric
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02249-4
Subject(s) - prolyl endopeptidase , t cell , microbiology and biotechnology , neprilysin , biology , programmed cell death , cytotoxic t cell , chemistry , immune system , immunology , biochemistry , apoptosis , in vitro , enzyme
Prolyl endopeptidase (PEP) is widely distributed and thought to play an important role in the degradation of peptide hormones and neuropeptides, but its biological role is totally unknown. In this study, we examined PEP activity in subpopulations of murine T cells and found that PEP activity was significantly higher in immature thymocytes than in mature thymocytes or in peripheral T cells. Stimulation of murine peripheral T cells time‐dependently increased PEP activity. Although murine T cell hybridomas exhibited high PEP activity, the PEP activity was fully inhibited by treatment with PEP inhibitor. The pretreated T cells were found to be resistant to activation‐induced cell death (AICD). Similar results were obtained in murine thymocytes as well as in activated peripheral T cells. PEP activity in T cell hybridomas remained unchanged during AICD. These results suggest that T cells expressing high PEP activity are susceptible to ACID.