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NF‐κB and ERK cooperate to stimulate DNA synthesis by inducing ornithine decarboxylase and nitric oxide synthase in cardiomyocytes treated with TNF and LPS
Author(s) -
Tantini Benedetta,
Pignatti Carla,
Fattori Monia,
Flamigni Flavio,
Stefanelli Claudio,
Giordano Emanuele,
Menegazzi Marta,
Clô Carlo,
Caldarera Claudio Marcello
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)02222-6
Subject(s) - ornithine decarboxylase , nitric oxide synthase , mapk/erk pathway , nitric oxide , tumor necrosis factor alpha , kinase , ornithine decarboxylase antizyme , dna synthesis , polyamine , nf κb , chemistry , lipopolysaccharide , phosphorylation , extracellular , microbiology and biotechnology , signal transduction , biology , biochemistry , enzyme , endocrinology , dna
We previously reported that tumor necrosis factor‐α (TNF) and lipopolysaccharide (LPS) stimulate DNA synthesis in chick embryo cardiomyocytes (CM) via nitric oxide and polyamine biosynthesis. Here we show an involvement of nuclear factor‐κB (NF‐κB) in the induction of nitric oxide synthase (NOS) and ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis. In addition NF‐κB activation appears to favor survival of CM by reducing caspase activation. TNF and LPS also stimulate phosphorylation of extracellular signal‐regulated kinase (ERK), which is required for the changes in ODC and caspase activity, but not for NOS induction or NF‐κB activation. In conclusion, these results indicate that NF‐κB, in cooperation with ERK, plays a pivotal role in the growth stimulating effects of TNF and LPS, leading to the induction of both ODC and NOS and to the reduction of caspase activity.