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Lectin‐like oxidized low density lipoprotein receptor‐1 (LOX‐1) serves as an endothelial receptor for advanced glycation end products (AGE)
Author(s) -
Jono Tadashi,
Miyazaki Akira,
Nagai Ryoji,
Sawamura Tatsuya,
Kitamura Toshinori,
Horiuchi Seikoh
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03325-7
Subject(s) - glycation , scavenger receptor , bovine serum albumin , cd36 , receptor , chemistry , lipoprotein , low density lipoprotein , medicine , endocrinology , advanced glycation end product , phospholipid , antibody , biochemistry , immunology , biology , cholesterol , membrane
Advanced glycation end products (AGE) are known to serve as ligands for the scavenger receptors such as SR‐A, CD36 and SR‐BI. In the current study, we examined whether AGE is recognized by lectin‐like oxidized low density lipoprotein receptor‐1 (LOX‐1). Cellular binding experiments revealed that AGE‐bovine serum albumin (AGE‐BSA) showed the specific binding to CHO cells overexpressing bovine LOX‐1 (BLOX‐1), which was effectively suppressed by an anti‐BLOX‐1 antibody. Cultured bovine aortic endothelial cells also showed the specific binding for AGE‐BSA, which was suppressed by 67% by the anti‐BLOX‐1 antibody. Thus, LOX‐1 is identified as a novel endothelial receptor for AGE.