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Evidence for cardiac sodium–calcium exchanger association with caveolin‐3
Author(s) -
Bossuyt Julie,
Taylor Bonnie E.,
James-Kracke Marilyn,
Hale Calvin C.
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03323-3
Subject(s) - vesicle , chemistry , caveolin , caveolae , sodium , sodium calcium exchanger , lipid raft , biophysics , caveolin 1 , biochemistry , membrane , intracellular , biology , organic chemistry
The interaction of cardiac Na + –Ca 2+ exchange (NCX1) with caveolin proteins was investigated in sarcolemmal vesicles. Western blots of sarcolemmal vesicles revealed the presence of caveolin‐1, ‐2, and ‐3. NCX1 co‐fractionated more closely with caveolin‐3 than caveolin‐1 on sucrose density gradients. NCX1 has five possible caveolin‐binding motifs and NCX1 co‐precipitated specifically with caveolin‐3. Molecular sieve column chromatography indicated that this co‐precipitation was not due to incomplete solubilization of lipid raft microdomains. Cholesterol chelation in vesicles decreased NCX1 transport activity and caveolin‐3 co‐precipitation. NCX1 may play a role in caveolar transmembrane signaling in addition to its role in excitation–contraction coupling.