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Identification of functional type 1 ryanodine receptors in mouse dendritic cells
Author(s) -
O'Connell Peta J.,
Klyachko Vitaly A.,
Ahern Gerard P.
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03321-x
Subject(s) - ryanodine receptor , dantrolene , microbiology and biotechnology , chemistry , receptor , intracellular , inositol , biochemistry , biology , calcium , organic chemistry
Ca 2+ signaling plays an important role in the function of dendritic cells (DC), the specialized antigen‐presenting cells of the immune system. Here we describe functional ryanodine receptor (RyR) Ca 2+ release channels in murine, bone marrow‐derived DC. RT‐PCR analysis identified selective expression of the type 1 RyR, with higher levels detected in immature rather than mature DC. The RyR activators caffeine, FK506, ryanodine and 4‐chloro‐ m ‐cresol mobilized Ca 2+ in DC, and responses to 4‐chloro‐ m ‐cresol were inhibited by dantrolene. Furthermore, activation of RyRs both inhibited subsequent inositol trisphosphate‐mediated Ca 2+ release and provoked store‐operated Ca 2+ entry, suggesting a functional interaction between these intracellular Ca 2+ channels. Thus, the RyR1 channel may play an intrinsic role in Ca 2+ signaling in DC.