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Conversion of L ‐tryptophan to serotonin and melatonin in human melanoma cells
Author(s) -
Slominski Andrzej,
Semak Igor,
Pisarchik Alexander,
Sweatman Trevor,
Szczesniewski Andre,
Wortsman Jacobo
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03319-1
Subject(s) - melatonin , serotonin , tryptophan , tryptophan hydroxylase , enzyme , chemistry , biochemistry , methyltransferase , melanoma , endocrinology , medicine , microbiology and biotechnology , biology , gene , methylation , cancer research , amino acid , receptor , serotonergic
We showed in human melanoma cells tryptophan hydroxylase (TPH) and hydroxyindole methyltransferase genes expression with the sequential enzymatic activities of TPH, serotonin (Ser) N ‐acetyltransferase and hydroxyindole methyltransferase. The presence of the products Ser, 5OH‐tryptophan, N ‐acetylserotonin, melatonin (Mel), 5‐methoxytryptamine and 5‐methoxytryptophol was documented by liquid chromatography–mass spectrometry. Thus, human melanoma cells can synthesize and metabolize Ser and Mel.