Premium
Sendai virus C protein impairs both phosphorylation and dephosphorylation processes of Stat1
Author(s) -
Komatsu Takayuki,
Takeuchi Kenji,
Yokoo Junko,
Gotoh Bin
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03301-4
Subject(s) - phosphorylation , dephosphorylation , stat1 , tyrosine phosphorylation , sendai virus , protein phosphorylation , tyrosine , interferon , protein tyrosine phosphatase , signal transduction , biology , microbiology and biotechnology , chemistry , biochemistry , virus , virology , phosphatase , protein kinase a
Sendai virus expresses C protein that blocks interferon (IFN) signaling. We previously reported suppression of IFN‐stimulated tyrosine phosphorylation of signal transducers and activators of transcription (Stats) in infected cells. However this conclusion has remained controversial. To settle it, we re‐examined the effect of C protein expression on phosphorylation of Stat1 in detail. IFN‐stimulated tyrosine phosphorylation of Stat1 was doubtlessly suppressed early in infection, but the suppression was incomplete, suggesting the importance of the unknown blocking mechanism that inactivates the tyrosine‐phosphorylated (pY)‐Stat1 generated as the signaling leak. Interestingly, the dephosphorylation process of pY‐Stat1 was also impaired. These effects on both phosphorylation and dephosphorylation processes were attributable to the function of the C protein.