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The N‐terminal domain of SV40 large T antigen represses the HER2/neu‐mediated transformation and metastatic potential in breast cancers
Author(s) -
Chuang Tzu-Chao,
Yu Yuh-Hua,
Lin Yen-Shing,
Wang Shan-Shue,
Kao Ming-Ching
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03277-x
Subject(s) - her2/neu , cancer research , oncogene , metastasis , antigen , metastatic breast cancer , sv40 large t antigen , breast cancer , cancer , in vitro , malignant transformation , biology , medicine , cell culture , immunology , transfection , cell cycle , biochemistry , genetics
HER2/neu is known to be overexpressed in approximately 40% of human breast and ovarian cancers and it is associated with increased metastasis and poor prognosis. We have shown previously that the N‐terminal domain of simian virus 40 large T antigen (LT425) can act as a transforming suppressor of the HER2 / neu oncogene in human ovarian cancer. In the present study, we demonstrate that LT425 can also repress the transforming properties of HER2/neu‐overexpressing human breast cancer cells. In addition, the results of a chemotaxis assay and an in vitro chemoinvasion assay further suggest that LT425 can also suppress the metastatic potential of the HER2/neu‐transformed breast cancer cells. Taken together, these data clearly suggest that the inhibition of the expression of p185 HER2/neu tyrosine kinase by LT425 is capable of suppressing the HER2/neu‐mediated transformation and metastatic potential in breast cancers.