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KCO1 is a component of the slow‐vacuolar (SV) ion channel
Author(s) -
Schönknecht Gerald,
Spoormaker Petra,
Steinmeyer Ralf,
Brüggeman Lioubov,
Ache Peter,
Dutta Rajiv,
Reintanz Birgit,
Godde Matthias,
Hedrich Rainer,
Palme Klaus
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03273-2
Subject(s) - vacuole , arabidopsis , green fluorescent protein , protoplast , patch clamp , arabidopsis thaliana , biophysics , ion channel , chemistry , wild type , fluorescence , microbiology and biotechnology , biology , cytoplasm , biochemistry , gene , mutant , physics , receptor , quantum mechanics
The Arabidopsis double pore K + channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT‐PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles from Arabidopsis wild‐type and kco1 knockout plants were isolated for patch‐clamp analyses. Currents mediated by slow‐activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild‐type. This shows that KCO1 is involved in the formation of SV channels.