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Expression and functional role of bTRPC1 channels in native endothelial cells
Author(s) -
Antoniotti Susanna,
Lovisolo Davide,
Fiorio Pla Alessandra,
Munaron Luca
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03256-2
Subject(s) - trpc1 , basic fibroblast growth factor , microbiology and biotechnology , transient receptor potential channel , receptor , gene isoform , chemistry , calcium , agonist , endocrinology , medicine , biology , growth factor , biochemistry , gene
We have analyzed the expression and localization of bovine transient receptor potential‐C1 (bTRPC1) in bovine aortic endothelial cells, and its possible involvement in the store‐independent calcium influx induced by basic fibroblast growth factor (bFGF). RT‐PCR experiments confirmed the existence of two btrpc1 mRNA isoforms; conversely, the btrpc3 gene was not transcribed. Anti‐TRPC1 antibody revealed the presence of the protein in the membrane‐rich compartment only. Application of anti‐TRPC1 during the response to bFGF caused a partial but significant reduction of calcium entry. This is the first evidence of TRP channel involvement in a non‐capacitative calcium influx induced by a biologically relevant agonist such as the angiogenic factor bFGF in native endothelial cells.