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Non‐antisense cellular responses to oligonucleotides
Author(s) -
Anselmet Alain,
Mayat Ebrahim,
Wietek Stefan,
Layer Paul G,
Payrastre Bernard,
Massoulié Jean
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03248-3
Subject(s) - oligonucleotide , internalization , phosphodiester bond , microbiology and biotechnology , chemistry , integrin , receptor , biochemistry , biophysics , biology , rna , dna , gene
Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double‐stranded or G‐quartet structures (ribo‐ or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10–20 h. This effect is reversible, non‐toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments.