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Progastrin processing differs in 7B2 and PC2 knockout animals: a role for 7B2 independent of action on PC2
Author(s) -
Rehfeld Jens F.,
Lindberg Iris,
Friis-Hansen Lennart
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03234-3
Subject(s) - knockout mouse , microbiology and biotechnology , action (physics) , chemistry , biology , biochemistry , receptor , physics , quantum mechanics
Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and α‐amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K 53 –K 54 site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin‐17), and that 7B2 exhibits effects beyond PC2‐mediated cleavages.