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NGF‐dependent and tissue‐specific transcription of vgf is regulated by a CREB–p300 and bHLH factor interaction
Author(s) -
Mandolesi Georgia,
Gargano Silvia,
Pennuto Maria,
Illi Barbara,
Molfetta Rosa,
Soucek Laura,
Mosca Laura,
Levi Andrea,
Jucker Richard,
Nasi Sergio
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03227-6
Subject(s) - creb , transcription factor , neurotrophin , biology , microbiology and biotechnology , basic helix loop helix , promoter , transcription (linguistics) , response element , regulation of gene expression , gene expression , gene , dna binding protein , genetics , receptor , linguistics , philosophy
Neurotrophins support neuronal survival, development, and plasticity through processes requiring gene expression. We studied how vgf target gene transcription is mediated by a critical promoter region containing E‐box, CCAAT and cAMP response element (CRE) sites. The p300 acetylase was present in two distinct protein complexes bound to this region. One complex, containing HEB (ubiquitous basic helix–loop–helix (bHLH)), bound the promoter in non‐neuronal cells and was involved in repressing vgf expression. Neurotrophin‐dependent transcription was mediated by the second complex, specific for neuronal cells, which included CRE binding protein and MASH1 (neuro‐specific bHLH), bound the CCAAT motif, and was target of neurotrophin signalling. The interaction, mediated by p300, of different transcription factors may add specificity to the neurotrophin response.