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Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non‐haem iron in human intestinal Caco‐2 cells
Author(s) -
Sharp Paul,
Tandy Sarah,
Yamaji Sachie,
Tennant Jason,
Williams Mark,
Singh Srai Surjit Kaila
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03225-2
Subject(s) - dmt1 , transporter , caco 2 , cytosol , biochemistry , chemistry , divalent , messenger rna , cell , ferrous , biology , enzyme , gene , organic chemistry
A divalent metal transporter, DMT1, located on the apical membrane of intestinal enterocytes is the major pathway for the absorption of dietary non‐haem iron. Using human intestinal Caco‐2 TC7 cells, we have shown that iron uptake and DMT1 protein in the plasma membrane were significantly decreased by exposure to high iron for 24 h, in a concentration‐dependent manner, whereas whole cell DMT1 protein abundance was unaltered. This suggests that part of the response to high iron involved redistribution of DMT1 between the cytosol and cell membrane. These events preceded changes in DMT1 mRNA, which was only decreased following 72 h exposure to high iron.