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Reactive oxygen species generated from the mitochondrial electron transport chain induce cytochrome c dissociation from beef‐heart submitochondrial particles via cardiolipin peroxidation. Possible role in the apoptosis
Author(s) -
Petrosillo Giuseppe,
Ruggiero Francesca Maria,
Pistolese Marilva,
Paradies Giuseppe
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03206-9
Subject(s) - cardiolipin , submitochondrial particle , cytochrome c , reactive oxygen species , chemistry , cytochrome , mitochondrion , coenzyme q – cytochrome c reductase , lipid peroxidation , biochemistry , electron transport chain , cytochrome p450 reductase , biophysics , biology , oxidative stress , membrane , enzyme , phospholipid
Cytochrome c release from mitochondria is a critical event in the apoptosis induction. Dissociation of cytochrome c from the mitochondrial inner membrane (IMM) is a necessary first step for cytochrome c release. In the present study, the effect of reactive oxygen species (ROS) on the dissociation of cytochrome c from beef‐heart submitochondrial particles (SMP) and on the cardiolipin content was investigated. Exposure of SMP to mitochondrial‐mediated ROS generation resulted in a large dissociation of cytochrome c from SMP and in a parallel loss of cardiolipin. Both these effects were directly and significantly correlated and also abolished by superoxide dismutase+catalase. These results demonstrate that ROS generation induces the dissociation of cytochrome c from IMM via cardiolipin peroxidation. The data may prove useful in clarifying the molecular mechanism underlying the release of cytochrome c from the mitochondria to the cytosol.