Premium
Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support
Author(s) -
Katayama Kazuhiro,
Dobashi Yoh,
Kitagawa Masatoshi,
Kamekura Soichiro,
Kawai Masataka,
Kadoya Yuichi,
Kameya Toru
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03200-8
Subject(s) - cyclin dependent kinase , cyclin d1 , microbiology and biotechnology , cyclin , cyclin d , cyclin a , apoptosis , cyclin b , cell cycle , kinase , cyclin d3 , biology , cancer research , cyclin dependent kinase 4 , cyclin e , transfection , flow cytometry , cyclin dependent kinase 2 , cell culture , protein kinase a , biochemistry , genetics
The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin‐dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1‐arrest. Cdk4 kinase activity was significantly higher in cdk4‐, or cyclin D1‐expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co‐transfection of p16 INK4 , or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.