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Sarcolemma phospholipid structure investigated by immunogold electron microscopy and 31 P NMR spectroscopy with lanthanide ions
Author(s) -
Moreau Céline,
Cavalier Annie,
Le Floch Marie,
Segalen Jacqueline,
Rocher Chantal,
Traı̈kia Mounir,
Leray Geneviève,
Bondon Arnaud,
Thomas Daniel,
Le Rumeur Elisabeth
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03199-4
Subject(s) - chemistry , phospholipid , nuclear magnetic resonance spectroscopy , vesicle , membrane , lipid bilayer , sarcolemma , magic angle spinning , spectroscopy , crystallography , analytical chemistry (journal) , nuclear magnetic resonance , chromatography , biochemistry , stereochemistry , physics , quantum mechanics
The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron microscopy and 31 P nuclear magnetic resonance (NMR) spectroscopy on isolated sarcolemma vesicles, we find that (i) the sarcolemma vesicles maintain the in‐vivo cellular sidedness, (ii) the phospholipid mobility is close to that observed in model membranes (similar lateral diffusion coefficients and spin–lattice T 1 relaxation times). Using broad‐band and magic angle spinning 31 P NMR spectroscopy with lanthanide ions (Pr 3+ ), it is possible to quantify the distribution of phospholipids between internal and external membrane layers, showing that the trans‐bilayer distribution is highly asymmetrical.