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Rapid increase of NO release in plant cell cultures induced by cytokinin
Author(s) -
Tun Ni N,
Holk André,
Scherer Günther F.E
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03164-7
Subject(s) - cytokinin , arabidopsis , plant cell , signal transduction , biochemistry , scavenger , chemistry , microbiology and biotechnology , biology , auxin , gene , mutant , radical
4,5‐Diaminofluorescein, a fluorescence indicator for NO, was applied to detect the release of NO from plant cells. NO production was increased within 3 min when plant cell cultures ( Arabidopsis , parsley, and tobacco) were treated by cytokinin and was dose‐dependent and signal‐specific in that other plant hormones and inactive cytokinin analog were not effective in stimulating of NO release. The response was quenched by addition of 2‐(aminoethyl)‐2‐thiopseudourea, an inhibitor of the animal NO synthase, and by addition of an NO scavenger, 2‐(4‐carboxyphenyl)‐4,4,5,5‐tetramethylimidazoline‐1‐1‐oxy‐3‐oxide. These results imply that NO may act in cytokinin signal transduction.