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BMP signaling regulates Nkx2‐5 activity during cardiomyogenesis
Author(s) -
Jamali Mina,
Karamboulas Christina,
Rogerson Parker J,
Skerjanc Ilona S
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03151-9
Subject(s) - microbiology and biotechnology , chemistry , signal transduction , business , biology
Nkx2‐5 regulates the transcription of muscle‐specific genes during cardiomyogenesis. Nkx2‐5 expression can induce cardiomyogenesis in aggregated P19 cells but not in monolayer cultures. In order to investigate the mechanism by which cellular aggregation regulates Nkx2‐5 function, we examined the role of bone morphogenetic protein 4 (BMP4). We showed that the expression of the BMP inhibitor, noggin, was sufficient to inhibit the induction of cardiomyogenesis by Nkx2‐5 during cellular aggregation. Furthermore, soluble BMP4 could activate Nkx2‐5 function in monolayer cultures, resulting in the formation of cardiomyocytes. Therefore, BMP signaling is necessary and sufficient for the regulation of Nkx2‐5 activity during cardiomyogenesis in P19 cells.