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Effect of D609 on phosphatidylcholine metabolism in the nuclei of LA‐N‐1 neuroblastoma cells: a key role for diacylglycerol
Author(s) -
Antony Pierre,
Farooqui Akhlaq A.,
Horrocks Lloyd A.,
Freysz Louis
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03149-0
Subject(s) - diacylglycerol kinase , phosphatidylcholine , phosphocholine , phospholipase c , phospholipase , biochemistry , chemistry , diglyceride , phospholipid , phospholipase d , enzyme , protein kinase c , membrane
In our previous studies, TPA treatment of LA‐N‐1 cells stimulated the production of diacylglycerol in nuclei, probably through the activation of a phospholipase C. Stimulation of the synthesis of nuclear phosphatidylcholine by the activation of CTP:phosphocholine cytidylyltransferase was also observed. The present data show that both effects were inhibited by the pretreatment of the cells with D609, a selective phosphatidylcholine‐phospholipase C inhibitor, indicating that the diacylglycerol produced through the hydrolysis of phosphatidylcholine in the nuclei is reutilized for the synthesis of nuclear phosphatidylcholine and is required for the activation of CTP:phosphocholine cytidylyltransferase.