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Reversible inhibition of hypoxia‐inducible factor 1 activation by exposure of hypoxic cells to the volatile anesthetic halothane
Author(s) -
Itoh Tatsuya,
Namba Tsunehisa,
Fukuda Kazuhiko,
Semenza Gregg L.,
Hirota Kiichi
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03119-2
Subject(s) - halothane , hypoxia (environmental) , erythropoiesis , hypoxia inducible factor 1 , transcription factor , hypoxia inducible factors , anesthetic , microbiology and biotechnology , hif1a , chemistry , angiogenesis , biology , gene , biochemistry , medicine , anesthesia , cancer research , oxygen , organic chemistry , anemia
Volatile anesthetics modulate a variety of physiological and pathophysiological responses including hypoxic responses. Hypoxia‐inducible factor 1 (HIF‐1) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O 2 availability in mammals, including erythropoiesis, angiogenesis, and glycolysis. We demonstrate for the first time that the volatile anesthetic halothane blocks HIF‐1 activity and downstream target gene expressions induced by hypoxia in the human hepatoma‐derived cell line, Hep3B. Halothane reversibly blocks hypoxia‐induced HIF‐1α protein accumulation and transcriptional activity at clinically relevant doses.