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Crystal structure of a cyclic form of bovine pancreatic trypsin inhibitor
Author(s) -
Botos Istvan,
Wu Zhibin,
Lu Wuyuan,
Wlodawer Alexander
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03113-1
Subject(s) - trypsin , crystal structure , chemistry , recombinant dna , mutant , trypsin inhibitor , crystallography , biochemistry , enzyme , gene
The crystal structure of a cyclic form of a mutant of bovine pancreatic trypsin inhibitor has been solved at 1.0 Å resolution. The protein was synthesized by native chemical ligation and its structure is almost indistinguishable from the previously described recombinant form of the same mutant; however, the new loop containing the former termini became much better ordered.