Premium
Paternally inherited transgenes are down‐regulated but retain low activity during early embryogenesis in Arabidopsis
Author(s) -
Baroux Célia,
Blanvillain Robert,
Gallois Patrick
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03097-6
Subject(s) - transactivation , arabidopsis , transgene , biology , gene , genetics , reporter gene , gus reporter system , arabidopsis thaliana , chimeric gene , gene expression , microbiology and biotechnology , genetically modified crops , mutant
We investigated the timing of transgene activation after fertilisation in Arabidopsis following crosses and using two transgenic promoters (from the AtCYCB1 and AtLTP1 genes). Using both a transactivation system and direct transcriptional fusion to drive β‐glucuronidase reporter expression, reciprocal crosses showed a lack of expression of the paternal components. This is consistent with a lack of paternal genome activity previously reported during early seed development in Arabidopsis [Viella‐Calzada et al. (2000) Nature 404, 91–94]. However, transactivation experiments of the BARNASE gene gave evidence that at least some paternal loci retain transcriptional activity, though at a low level, during early embryogenesis.