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Nucleoside diphosphate kinase III is localized to the inter‐membrane space in plant mitochondria
Author(s) -
Sweetlove L.J,
Mowday B,
Hebestreit H.F,
Leaver C.J,
Millar A.H
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03069-1
Subject(s) - nucleoside diphosphate kinase , biochemistry , mitochondrion , arabidopsis , nucleoside , kinase , cell fractionation , arabidopsis thaliana , chemistry , biology , fractionation , membrane , microbiology and biotechnology , gene , chromatography , mutant
Three types of nucleoside diphosphate kinases (NDPKs) are found in plants but the intra‐cellular compartmentation of these proteins is not certain, especially the location of the recently identified type III proteins. Through the fractionation of plant mitochondria from potato and Arabidopsis , display of protein profiles by 2D gel electrophoresis, and identification by mass spectrometry, we present the first direct evidence that type III proteins are localized in the inter‐membrane space of plant mitochondria. The possible metabolic functions of NDPK III are discussed in light of its sub‐cellular localization.