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Involvement of phospholipase D in oxidative stress‐induced necrosis of vascular smooth muscle cells
Author(s) -
Shin Eun-Young,
Min Do Sik,
Shin Ji-Cheol,
Shin Kyung-Sun,
Hyun Min-Soo,
Ha Kwon-Soo,
Kim Hun-Sik,
Ahn Hee-Yul,
Kim Eung-Gook
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03059-9
Subject(s) - necrosis , oxidative stress , vascular smooth muscle , extracellular , phospholipase d , chemistry , hydrogen peroxide , intracellular , phospholipase , tumor necrosis factor alpha , endocrinology , microbiology and biotechnology , biochemistry , medicine , biology , enzyme , smooth muscle
Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress‐induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus ortho vanadate) but not hydrogen peroxide alone activated PLD in a dose‐ and time‐dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan‐1‐ol, a PLD inhibitor, attenuated both pervanadate‐induced necrosis and increase of intracellular Ca 2+ . Removal of extracellular Ca 2+ inhibited pervanadate‐induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate‐induced necrosis of VSMCs, which is at least partly due to Ca 2+ toxicity.