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Neutral fluorescence probe with strong ratiometric response to surface charge of phospholipid membranes
Author(s) -
Duportail Guy,
Klymchenko Andrey,
Mely Yves,
Demchenko Alexander
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03055-1
Subject(s) - phospholipid , fluorescence , chemistry , membrane , bilayer , lipid bilayer , chromophore , phosphatidylcholine , photochemistry , excited state , liposome , cationic polymerization , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , physics , quantum mechanics , nuclear physics
We report on dramatic differences in fluorescence spectra of 4′‐dimethylamino‐3‐hydroxyflavone (probe F) studied in phospholipid membranes of different charge (phosphatidyl glycerol, phosphatidylcholine (PC), their mixture and the mixture of PC with a cationic lipid). The effect consists in variations of relative intensities at two well‐separated band maxima at 520 and 570 nm belonging to normal (N*) and tautomer (T*) excited states of flavone chromophore. Based on these studies we propose a new approach to measure electrostatic potential at the surface layer of phospholipid membranes, which is based on potential‐dependent changes of bilayer hydration and involves very sensitive and convenient ratiometric measurements in fluorescence emission.