Premium
Induction of H 2 O 2 synthesis by β‐glucan elicitors in soybean is independent of cytosolic calcium transients
Author(s) -
Mithöfer Axel,
Fliegmann Judith,
Daxberger Andrea,
Ebel Chantal,
Neuhaus-Url Gabriele,
Bhagwat Arvind A,
Keister Donald L,
Ebel Jürgen
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03054-x
Subject(s) - cytosol , calcium , chemistry , glucan , biochemistry , enzyme , organic chemistry
Soybean cell suspension cultures have been used to investigate the role of the elevation of the cytosolic Ca 2+ concentration in β‐glucan elicitors‐induced defence responses, such as H 2 O 2 and phytoalexin production. The intracellular Ca 2+ concentration was monitored in transgenic cells expressing the Ca 2+ ‐sensing aequorin. Two lines of evidence showed that a transient increase of the cytosolic Ca 2+ concentration is not necessarily involved in the induction of H 2 O 2 generation: (i) a Bradyrhizobium japonicum cyclic β‐glucan induced the H 2 O 2 burst without increasing the cytosolic Ca 2+ concentration; (ii) two ion channel blockers (anthracene‐9‐carboxylate, A9C; 5‐nitro‐2‐(3‐phenylpropylamino)‐benzoate, NPPB) could not prevent a Phytophthora soja β‐glucan elicitor‐induced H 2 O 2 synthesis but did prevent a cytosolic Ca 2+ concentration increase. Moreover, A9C and NPPB inhibited P. sojae β‐glucan‐elicited defence‐related gene inductions as well as the inducible accumulation of phytoalexins, suggesting that the P. sojae β‐glucan‐induced transient cytosolic Ca 2+ increase is not necessary for the elicitation of H 2 O 2 production but is very likely required for phytoalexin synthesis