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P34 cdc2 kinase is associated with cortical microtubules from higher plant protoplasts
Author(s) -
Hemsley Rachel,
McCutcheon Sandra,
Doonan John,
Lloyd Clive
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03046-0
Subject(s) - phragmoplast , immunogold labelling , microtubule , microbiology and biotechnology , cell plate , interphase , chemistry , plant cell , biology , biophysics , spindle apparatus , cytokinesis , biochemistry , cell , botany , cell division , ultrastructure , gene
The cell cycle regulatory enzyme p34 cdc2 kinase is known to be localized to the preprophase band, the spindle and the phragmoplast, but not to interphase cortical microtubules. This was investigated further by mechanically cleaving substrate‐attached protoplasts to leave plasma membrane disks bearing microtubules freed of nuclear and cytosolic signal. Antibodies to PSTAIRE and to specific C‐terminal peptides of cdc2a, were used in immunofluorescence, protein blotting and immunogold electron microscopy to demonstrate that antigen is located on the cortical microtubules of carrot, tobacco BY‐2 and Arabidopsis cells.