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Geraniol 10‐hydroxylase 1 , a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis
Author(s) -
Collu Graziella,
Unver Nehir,
Peltenburg-Looman Anja M.G.,
van der Heijden Robert,
Verpoorte Robert,
Memelink Johan
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03045-9
Subject(s) - catharanthus roseus , biochemistry , biosynthesis , terpenoid , indole alkaloid , biology , vindoline , cytochrome p450 , enzyme , indole test , vinblastine , genetics , chemotherapy
Geraniol 10‐hydroxylase (G10H) is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. Catharanthus roseus (Madagascar periwinkle) contains monoterpenoid indole alkaloids, several of which are pharmaceutically important. Vinblastine and vincristine, for example, find widespread use as anti‐cancer drugs. G10H is thought to play a key regulatory role in terpenoid indole alkaloid biosynthesis. We purified G10H from C. roseus cells. Using degenerate PCR primers based on amino acid sequence information we cloned the corresponding cDNA. The encoded CYP76B6 protein has G10H activity when expressed in C. roseus and yeast cells. The stress hormone methyljasmonate strongly induced G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in a C. roseus cell culture.