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Effect of human recombinant leptin on lipid handling by fully differentiated Caco‐2 cells
Author(s) -
Stan S,
Levy E,
Bendayan M,
Zoltowska M,
Lambert M,
Michaud J,
Asselin C,
Delvin E.E
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03032-0
Subject(s) - chylomicron , leptin , apolipoprotein b , chemistry , endocrinology , medicine , lipoprotein , leptin receptor , caco 2 , receptor , ldl receptor , cholesterol , biochemistry , biology , very low density lipoprotein , cell , obesity
It has been established that leptin displays a number of effects on peripheral tissues. We have investigated the effect of the hormone on lipid synthesis, apolipoprotein biogenesis and lipoprotein secretion in Caco‐2 cells. Immunocytochemistry revealed the presence of leptin receptors (Ob‐Rb) on the basolateral membrane. Incubation of cells with 200 nM leptin resulted in a decreased export of triglycerides in the basolateral medium without affecting monoglyceride, diglyceride and cholesterol ester lipid classes. It also significantly reduced the output of de novo‐synthesized apolipoprotein (Apo)B‐100 and ApoB‐48 as well as that of newly formed chylomicrons and of low‐density lipoproteins. It also enhanced that of ApoA‐I, ApoA‐IV and ApoE. Our results support the hypothesis that leptin can affect energy balance at the gut level by reducing lipid release into the circulation.