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A pathogen‐inducible divinyl ether synthase ( CYP74D ) from elicitor‐treated potato suspension cells 1
Author(s) -
Stumpe Michael,
Kandzia Romy,
Göbel Cornelia,
Rosahl Sabine,
Feussner Ivo
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03019-8
Subject(s) - elicitor , phytophthora infestans , biochemistry , escherichia coli , linoleic acid , complementary dna , biology , pseudomonas syringae , metabolite , chemistry , fatty acid , enzyme , gene
In elicitor‐treated potato cells, 9‐lipoxygenase‐derived oxylipins accumulate with the divinyl ether colneleic acid as the major metabolite. Here, the identification of a potato cDNA is described, whose predicted amino acid sequence corresponds to divinyl ether synthases, belonging to the recently identified new P450 subfamily CYP74D. The recombinant protein was expressed in Escherichia coli and shown to metabolize 9‐hydroperoxy linoleic acid to colneleic acid at pH 6.5. This fatty acid derivative has been implicated in functioning as a plant antimicrobial compound. RNA blot analyses revealed accumulation of divinyl ether synthase transcripts both upon infiltration of potato leaves with Pseudomonas syringae and after infection with Phytophthora infestans .