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Involvement of CCAAT/enhancer‐binding protein in regulation of the rat serine:pyruvate/alanine:glyoxylate aminotransferase gene expression
Author(s) -
Sugiyama Tsuyoshi,
Uchida Chiharu,
Oda Toshiaki,
Kitagawa Masatoshi,
Hayashi Hideharu,
Ichiyama Arata
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03010-1
Subject(s) - microbiology and biotechnology , ccaat enhancer binding proteins , alanine , biology , promoter , peroxisome , serine , gene , transcription factor , transcription (linguistics) , enhancer , gene expression , biochemistry , dna binding protein , enzyme , amino acid , linguistics , philosophy
In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted to characterize the downstream promoter responsible for generation of peroxisomal SPT/AGT. The minimal downstream promoter was confined to the +21–+90 region. We demonstrated that C/EBPα and C/EBPβ bound around the downstream start site (+66) contribute to the promoter activity. The downstream promoter activity is also regulated positively by a short inverted repeat, located 20–30 bp upstream of the downstream start site, through a protein factor(s) bound to this region. On the other hand, the sequence just downstream of the start site may negatively regulate the promoter activity.