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Properties of the detergent solubilised cytochrome c oxidase (cytochrome cbb 3 ) purified from Pseudomonas stutzeri
Author(s) -
Urbani Andrea,
Gemeinhardt Sabine,
Warne Antony,
Saraste Matti
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03006-x
Subject(s) - pseudomonas stutzeri , cytochrome , chemistry , oxidase test , isoelectric point , cytochrome c oxidase , biochemistry , hemeprotein , stereochemistry , bacteria , heme , enzyme , biology , genetics
Cytochrome cbb 3 is a cytochrome c ‐oxidising isoenzyme that belongs to the superfamily of respiratory haem/copper oxidases. We have developed a purification method yielding large amounts of pure cbb 3 complex from the soil bacterium Pseudomonas stutzeri . This cytochrome cbb 3 complex consists of three subunits (ccoNOP) in a 1:1:1 stoichiometry and contains two b ‐type and three c ‐type haems. The protein complex behaves as a monomer with an overall molecular weight of 114.0±8.9 kDa and a s 0 20,w value of 8.9±0.3 S as determined by analytical ultracentrifugation. Crystals diffracting to 5.0 Å resolution have been grown by the vapour diffusion sitting drop method to an average size of 0.1×0.1×0.3 mm. This is the first crystallisation report of a ( cbb 3 )‐type oxidase.