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Interleukin‐1β stimulates cyclic GMP efflux in brain astrocytes
Author(s) -
Pedraza Carlos E,
Baltrons Marı́a Antonia,
Garcı́a Agustina
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)03003-4
Subject(s) - efflux , chemistry , stimulation , extracellular , verapamil , probenecid , interleukin , receptor , medicine , endocrinology , biochemistry , biology , cytokine , calcium , organic chemistry
In rat brain astroglia‐enriched cultures long‐term treatment with interleukin‐1β induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin‐1β effect is mediated by type I receptor and potentiated by interferon‐γ. In cells treated with bacterial endotoxin a larger NO‐dependent cGMP accumulation occurs only intracellularly, however a significant cGMP egression is observed when cells are co‐treated with interleukin‐1β. The non‐selective anion transport inhibitors probenecid and verapamil block cGMP efflux, indicating that interleukin‐1β stimulates a cGMP transporter.