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Inhibition by insulin of resistin gene expression in 3T3‐L1 adipocytes
Author(s) -
Haugen Fred,
Jørgensen Aud,
Drevon Christian A,
Trayhurn Paul
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02968-4
Subject(s) - resistin , endocrinology , medicine , insulin resistance , adipose tissue , 3t3 l1 , downregulation and upregulation , adipocyte , insulin , messenger rna , rosiglitazone , gene expression , adipogenesis , chemistry , biology , adipokine , gene , biochemistry
Expression of the gene encoding resistin, a low molecular weight protein secreted from adipose tissue postulated to link obesity and type II diabetes, was examined in 3T3‐L1 adipocytes. Resistin mRNA was detected in 3T3‐L1 cells by day 3 following induction of differentiation into adipocytes; by day 4 the level of resistin mRNA peaked and remained high. The PPARγ activators, rosiglitazone or darglitazone, reduced the level of resistin mRNA. Dexamethasone upregulated resistin mRNA level, but no effect was observed with the β 3 ‐adrenoceptor agonist, BRL 37344. A substantial reduction in resistin mRNA level was observed with insulin, which induced decreases at physiological concentrations. Insulin may be a major inhibitor of resistin production, and this does not support a role for resistin in insulin resistance.