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GM3 ganglioside inhibits endothelin‐1‐mediated signal transduction in C6 glioma cells
Author(s) -
Bassi Rosaria,
Viani Paola,
Giussani Paola,
Riboni Laura,
Tettamanti Guido
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02966-0
Subject(s) - ganglioside , signal transduction , microbiology and biotechnology , endothelin 1 , chemistry , phospholipase c , cell type , biology , glioma , cell , biochemistry , cancer research , receptor
We found that sparse and confluent C6 glioma cells differ both in GM3 content, which increases with cell density, and in endothelin‐1 (ET‐1)‐induced phosphoinositide hydrolysis, which was markedly higher in the sparse cells than in the confluent. Also after manipulation of the cellular GM3 content through treatment with exogenous GM3 or with drugs known to affect GM3 metabolism, the ET‐1 effect was inversely related to GM3 cellular levels. Cell treatment with an anti‐GM3 mAb resulted in the enhancement of ET‐1‐induced phospholipase C activation and restored the capacity of GM3‐treated cells to respond to ET‐1. These findings suggest that the GM3 ganglioside represents a physiological modulator of ET‐1 signaling in glial cells.