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Differential subcellular localization of hZip1 in adherent and non‐adherent cells
Author(s) -
Milon Béatrice,
Dhermy Didier,
Pountney David,
Bourgeois Monique,
Beaumont Carole
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02950-7
Subject(s) - subcellular localization , endoplasmic reticulum , microbiology and biotechnology , protein subcellular localization prediction , transfection , chemistry , intracellular , cell culture , nuclear localization sequence , biology , biochemistry , gene , cytoplasm , genetics
Two human divalent cation transporters of the ZIP family, hZip1 and hZip2, homologous to Irt1 ( Arabidopsis thaliana ), the first identified member, have been described. They were shown by transfection into K562 cells to be localized at the plasma membrane and to mediate zinc uptake. Here we report a differential subcellular localization of hZip1 according to cell type. By transient expressions of EGFP‐hZip1, FLAG‐tagged or native hZip1, we observed that hZip1 has a vesicular localization in COS‐7 cells or in several epithelial cell lines, corresponding partially to the endoplasmic reticulum. Using anti‐hZip1 antibodies, we confirmed the intracellular localization of the endogenous protein in PC‐3, a prostate cancer cell line.