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Organic phosphates as a new class of soluble guanylate cyclase inhibitors
Author(s) -
Suzuki Tsuneharu,
Suematsu Makoto,
Makino Ryu
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02943-x
Subject(s) - chemistry , gucy1a3 , biochemistry , gucy1b3 , enzyme , inositol phosphate , nitric oxide , inositol , guanylate cyclase 2c , cyclase , organic chemistry , receptor
This study aimed to examine effects of varied organic phosphates on activities of soluble guanylate cyclase (sGC). The enzyme was purified from bovine lung. Physiologically relevant concentrations of ATP, 2,3‐bisphosphoglyceric acid and inositol hexakisphosphate inhibited its enzyme activities under steady‐state conditions as well as those determined under stimulation with S ‐nitroso‐ N ‐acetylpenicillamine, a nitric oxide donor, carbon monoxide or YC‐1. Lineweaver–Burk plot analyses revealed that these three organic phosphates act as competitive inhibitors. Other organic phosphates such as cardiolipin and sphingomyelin but not inorganic phosphates exhibited such inhibitory actions. These results suggest that organic phosphates serve as inhibitors for sGC‐dependent signaling events.