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Elements from the cAMP signaling pathway are involved in the control of expression of the yeast gluconeogenic gene FBP1
Author(s) -
Zaragoza Oscar,
Gancedo Juana M
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02922-2
Subject(s) - catabolite repression , saccharomyces cerevisiae , schizosaccharomyces pombe , psychological repression , yeast , gene , biochemistry , fructose 1,6 bisphosphatase , gene expression , biology , fructose , chemistry , microbiology and biotechnology , mutant
cAMP represses the transcription of some Saccharomyces cerevisiae genes sensitive to catabolite repression. The effect of cAMP on the expression of FBP1 , encoding fructose‐1,6‐bisphosphatase (FbPase), has been further investigated. In yeast cells shifted to a derepressing medium, synthesis of FbPase was delayed if the strong decrease in intracellular cAMP, which occurs during the shift, was prevented. A similar delay occurred in a RAS2 val19 strain, while in a tpk1 w strain, with weak protein kinase A activity, induction of FbPase occurred earlier than in a TPK1 strain. In the tpk1 w strain, proteins which bind the UAS1 element of FBP1 were present during growth on glucose but they were only weakly operative. Expression of CAT8 and SIP4 , encoding proteins which bind the UAS2 element, was blocked by a high concentration of cAMP, but catabolite repression of these genes was not much relieved in a tpk1 w strain. We conclude that in S. cerevisiae , as reported for Schizosaccharomyces pombe , control of FBP1 requires both cAMP‐dependent and independent pathways; however, the mechanisms operating in the two yeasts are different.