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Role of Flk‐1 in mouse hematopoietic stem cells
Author(s) -
Haruta Hirotaka,
Nagata Yuka,
Todokoro Kazuo
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02921-0
Subject(s) - cd34 , haematopoiesis , stem cell , stem cell factor , biology , bone marrow , microbiology and biotechnology , adult stem cell , population , hematopoietic stem cell , endothelial stem cell , immunology , in vitro , medicine , genetics , environmental health
It was reported that human hematopoietic stem cells in bone marrow were restricted to the CD34 + KDR + cell fraction. We found that expression levels of Flk‐1, a mouse homologue of KDR, were low or undetectable in mouse Lin − c‐Kit + Sca‐1 + CD34 low/− cells as well as Hoechst33342 − cells (side population), which have long‐term reconstitution capacity. Furthermore, neither Flk‐1 + CD34 low/− cells nor Flk‐1 + CD34 + cells had long‐term reconstitution capacity in mouse. Taken together with other observations using Flk‐1‐deficient mice, these results indicate that Flk‐1 is essential for the development of hematopoietic stem cells in embryo but not for the function of hematopoietic stem cells in adult mouse bone marrow.