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Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae
Author(s) -
Roumanie Olivier,
Weinachter Caroline,
Larrieu Isabelle,
Crouzet Marc,
Doig François
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02906-4
Subject(s) - cdc42 , gtpase , saccharomyces cerevisiae , gtpase activating protein , microbiology and biotechnology , yeast , biology , biochemistry , chemistry , g protein , signal transduction
Rho proteins are down‐regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two‐hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stimulated the GTPase activity of the Rho1 protein, Lrg1p acted on the Cdc42 and Rho2 GTPases and we showed that Rgd2p has a GAP activity on both Cdc42p and Rho5p. In addition, we brought the first evidence for the existence of a sixth functional Rho in yeast, the Cdc42/Rac‐like GTPase Rho5.